ASATP

Tools:

ASRecovist

Alternative splicing recognition and visualization tool, which is a program to detect alternative splicing events from a gene annotation and classified them into different types (CE, IR, MXE, A3SS, A5SS, AFE, ALE and other). Alternative splicing events will be showed in tables and graphs. For example: anno.gtf

Paste Gtf Data:

Or Upload a Gtf File

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Output graph?

Yes No

Graph format?

SVG PNG

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ASQuantityDiff

Alternative splicing quantity comparison between samples, which was used to detect expression regulations of alternative transcripts. For example: isoform_exp.txt AS_event.xls AS_event_group.xls

Paste Transcript Expression Data:

Or Upload a File

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Upload "AS_event.xls or ASAffectORF_event.xls":

 

Upload "AS_event_group.xls":

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Q-value cutoff:

Transcript cutoff:

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ASAffectORF

Check AS event in CDS region, to identify the effect of alternative splicing event for ORFs. For example: anno.gtf AS_event.xls

Paste Gtf Data (with CDS type):

Or Upload a Gtf File

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Upload "AS_event.xls":

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runASATP

Run Alternative splicing Analysis Tool Package, which was a combined pipeline including ASRecovist, ASQuantityDiff and ASAffectORF. For example: anno.gtf isoform_exp.txt

Paste Gtf Data (with CDS type):

Or Upload a Gtf File

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Paste Transcript Expression Data:

Or Upload a File

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Output graph?

Yes No

Graph format?

SVG PNG

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asp2bit

Transform AS pattern to bit matrix. For example: 16

ASP code:

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Output graph?

Yes No

Graph format?

SVG PNG

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bit2asp

Transform bit to ASP code. For example: 10001,--101

Bit Matrix:

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Output graph?

Yes No

Graph format?

SVG PNG

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Databases:

Organism Query

Query genes with alternative splicing in model organisms. Gene structures are from different database, i.e. Ensembl (Homo_sapiens, Mus_musculus), WormBase (Caenorhabditis_elegans), FlyBase (Drosophila_melanogaster), TAIR (Arabidopsis_thaliana).

For example: ENSG00000101448 (Homo_sapiens), ENSMUSG00000108106 (Mus_musculus), AT5G66720 (Arabidopsis_thaliana), WBGene00235129 (Caenorhabditis_elegans), FBgn0267726 (Drosophila_melanogaster).

Organism select:

Homo_sapiens Mus_musculus Drosophila_melanogaster Caenorhabditis_elegans Arabidopsis_thaliana

Gene Id: